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Benchmark Specialty Agarose: Low Melt, Hi-Res, 3:1, Low EEO

 

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Benchmark Specialty Agarose: Low Melt, Hi-Res, 3:1, Low EEO

Benchmark's Specialty Agarose is the perfect choice when resolving small DNA/RNA fragments, isolating nucleic acids from gels or performing in-gel manipulations. Available in three grades, LM, HR and 3:1, it will meet all your electrophoretic needs.

Agarose LM - this low melt agarose can be used to resolve samples from 200bp to 25kb. It is perfect for prep gels as it has a melting point well below denaturation
temperatures (65°C). The agarose remains liquid at 37°C, allowing for procedures such as restriction digests and labeling to be carried out in the gel.

Agarose HR - for applications such as analysis of STR's, AFLP's, and PCR products where separation of small fragments is required, Benchmark's Agarose HR is ideal. Clear resolution of 20bp to 800bp fragments can be carried out in this intermediate melting agarose.

Agarose 3:1 - this blended high resolution agarose is designed for separation of fragments less than 1,000bp. Gels made with Agarose 3:1 are suitable for analytical and blotting applications.

Agarose LE - is a highly purified agarose, suitable for a variety of molecular biology applications. It is refined using an advanced process that excludes the use of organic solvents, yielding a cleaner end-product with a significantly reduced environmental impact. Agarose LE can be used for analyses of proteins and nucleic acids of various sizes (150bp to 6kbp).

All Benchmark agarose is manufactured under a "green" process that eliminates the use of organic solvents. They provide gels with enhanced resolution and clarity, low EEO and low backgrounds. All the agarose is tested to be RNase, DNase, Protease and Endonuclease free.

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  • Agarose LM, Low melting point (< 65°C)
  • Agarose HR, PCR grade for DNA fragments between 20-800 bp
  • Agarose 3:1, Blended agarose for small fragments
  • Agarose LE, Low EEO / increase electrophoretic mobility
  Agarose LM Agarose HR Agarose 3:1 Agarose LE
Gel Strength > 200g/cm² (1%) > 750g/cm² (1.5%) > 650g/cm² (1.5%) > 1200g/cm² (1%)
Gel Temperature 26°C to 30°C ≤ 33°C ≤ 36°C 36°C &plusm;1.5°C
Melt temperature ≤ 65°C ≤ 70°C ≤ 80°C 88°C &plusm;1.5°C
EEO (-mr) ≤ 0.1% ≤ 0.1% ≤ 0.1% ≤ 0.13%
Moisture Content ≤ 10% ≤ 10% ≤ 10% ≤ 7%
Sulfate ≤ 0.1% ≤ 0.1% ≤ 0.1% ≤ 0.2%
RNase/DNase None Detected None Detected None Detected None Detected
Protease/Endonuclease None Detected None Detected None Detected None Detected
Storeage Conditions Room Temperature Room Temperature Room Temperature Room Temperature
Resolution 200bp to 25kbp 20bp to 800bp 50bp to 1kb 150bp to 6kbp
Applications Prep gels
In Gel Processing
AFLP's STR's
PCR Small Fragments
PCR Blotting Multi-purpose, high purity

Ordering Info

Cat No Vendor Part No Description Qty Price Qty UOM
E166-3 A1801-LM Agarose LM, Low Melt Agarose, 100g In Stock 1 $388.41 EA
E166-4 A1801-HR Agarose HR, Hi-Res Agarose, 100g In Stock 1 $335.88 EA
E166-5 A1801-31 Agarose 3:1, Blended Agarose, 100g In Stock 1 $295.61 EA
E166-6 A1705 Agarose LE, Low EEO, 500g In Stock 1 $487.40 EA
 

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